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KMID : 1005219930080020143
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Journal of Embryo Transfer
1993 Volume.8 No. 2 p.143 ~ p.149
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Birth of Calf Following Transfer of Bovine Embryos Produced by Maturation, Fertilization and Development In Vitro with Korean Native Cattle Semen
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황우석
조충호/이병천/신태영/노상호/김성기/전병준/이강남/신언익
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Abstract
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The objective of this study was to produce calves derived from in vitro fertilization of in vitro matured follicular oocytes. Oocytes aspirated from small antral folicles of ovaries obtained at a local slaughter house were matured and fertilized in vitro. At l8hrs after insemination with Korean native cattle semen, oocytes were co-cultured for 6~7 days by utilizing co-culture system with bovine oviduct epithelial cell. After co-culture, good or excellent quality late morulae or early blastocysts were selected by morphological criteria under stereo microscope. Selected embryos were transferred to recipients on day 6 or 7 (estrus = day 0). Recipients were monitored by observation for estrus and rectal palpation after 60 days from embryo transfer. One of them went to term with the birth of a calf. This case is the first production of calf derived from in vitro fertilization in Korea.
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